Optimization of PCR Conditions for Amplification of GC‐Rich EGFR Promoter Sequence

BACKGROUND: Polymerase chain reaction (PCR) is an extremely sensitive method that often demands optimization, especially when difficult templates need to be amplified. The aim of the present study was to optimize the PCR conditions for amplification of the epidermal growth factor receptor (EGFR) pro...

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Bibliographische Detailangaben
Veröffentlicht in:J Clin Lab Anal
Hauptverfasser: Obradovic, Jasmina, Jurisic, Vladimir, Tosic, Natasa, Mrdjanovic, Jasminka, Perin, Branislav, Pavlovic, Sonja, Djordjevic, Natasa
Format: Artigo
Sprache:Inglês
Veröffentlicht: John Wiley and Sons Inc. 2013
Schlagworte:
Original Articles
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6807403/
https://ncbi.nlm.nih.gov/pubmed/24218132
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jcla.21632
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