Optimization of PCR Conditions for Amplification of GC‐Rich EGFR Promoter Sequence

BACKGROUND: Polymerase chain reaction (PCR) is an extremely sensitive method that often demands optimization, especially when difficult templates need to be amplified. The aim of the present study was to optimize the PCR conditions for amplification of the epidermal growth factor receptor (EGFR) pro...

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Detalhes bibliográficos
Publicado no:J Clin Lab Anal
Main Authors: Obradovic, Jasmina, Jurisic, Vladimir, Tosic, Natasa, Mrdjanovic, Jasminka, Perin, Branislav, Pavlovic, Sonja, Djordjevic, Natasa
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2013
Assuntos:
Original Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6807403/
https://ncbi.nlm.nih.gov/pubmed/24218132
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/jcla.21632
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