Viability Quantitative PCR Utilizing Propidium Monoazide, Spheroplast Formation, and Campylobacter coli as a Bacterial Model

A viability quantitative PCR (qPCR) utilizing propidium monoazide (PMA) is presented for rapid quantification of viable cells using the foodborne pathogen Campylobacter coli as a bacterial model. It includes optimized spheroplast formation via lysozyme and EDTA, induction of a mild osmotic shock for...

Description complète

Enregistré dans:
Détails bibliographiques
Publié dans:Appl Environ Microbiol
Auteurs principaux: Lazou, Thomai P., Iossifidou, Eleni G., Gelasakis, Athanasios I., Chaintoutis, Serafeim C., Dovas, Chrysostomos I.
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 2019
Sujets:
Methods
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6805072/
https://ncbi.nlm.nih.gov/pubmed/31420339
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01499-19
Tags: Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!

Documents similaires