Viability Quantitative PCR Utilizing Propidium Monoazide, Spheroplast Formation, and Campylobacter coli as a Bacterial Model

A viability quantitative PCR (qPCR) utilizing propidium monoazide (PMA) is presented for rapid quantification of viable cells using the foodborne pathogen Campylobacter coli as a bacterial model. It includes optimized spheroplast formation via lysozyme and EDTA, induction of a mild osmotic shock for...

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Bibliografische gegevens
Gepubliceerd in:Appl Environ Microbiol
Hoofdauteurs: Lazou, Thomai P., Iossifidou, Eleni G., Gelasakis, Athanasios I., Chaintoutis, Serafeim C., Dovas, Chrysostomos I.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: American Society for Microbiology 2019
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6805072/
https://ncbi.nlm.nih.gov/pubmed/31420339
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01499-19
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