Determination of the viability of Toxoplasma gondii oocysts by PCR real-time after treatment with propidium monoazide

This study aimed to investigate a methodology for discriminating viable and non-viable T. gondii oocysts in water. Analyses included two steps: (i) microscopic investigation with vital dyes; (ii) molecular investigation, using a real time PCR (qPCR), after parasite treatment (or not) with propidium...

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Veröffentlicht in:Rev Inst Med Trop Sao Paulo
Hauptverfasser: Marciano, Maria Aparecida Moraes, Silva, Rafaela Aparecida, Barbosa, Maria Luisa, Ferreira, Antônio Roberto Souza, Pereira-Chioccola, Vera Lucia
Format: Artigo
Sprache:Inglês
Veröffentlicht: Instituto de Medicina Tropical 2020
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Original Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7608075/
https://ncbi.nlm.nih.gov/pubmed/33146312
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1590/S1678-9946202062084
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