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A UV/Vis Spectroscopy-Based Assay for Monitoring of Transformations Between Nucleosides and Nucleobases

Efficient reaction monitoring is crucial for data acquisition in kinetic and mechanistic studies. However, for conversions of nucleosides to their corresponding nucleobases, as observed in enzymatically catalyzed nucleoside phosphorylation reactions, the current analytical arsenal does not meet mode...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Methods Protoc
Hauptverfasser: Kaspar, Felix, Giessmann, Robert T., Krausch, Niels, Neubauer, Peter, Wagner, Anke, Gimpel, Matthias
Format: Artigo
Sprache:Inglês
Veröffentlicht: MDPI 2019
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6789650/
https://ncbi.nlm.nih.gov/pubmed/31311105
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/mps2030060
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