Identification of a crucial amino acid implicated in the hydroxylation/desaturation ratio of CpFAH12 bifunctional hydroxylase

Claviceps purpurea bifunctional Δ12‐hydroxylase/desaturase, CpFAH12, and monofunctional desaturase CpFAD2, share 86% of sequence identity. To identify the underlying determinants of the hydroxylation/desaturation specificity, chimeras of these two enzymes were tested for their fatty acid production...

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Bibliographic Details
Published in:Biotechnol Bioeng
Main Authors: Robin, Julien, Gueroult, Marc, Cheikhrouhou, Randa, Guicherd, Marie, Borsenberger, Vinciane, Marty, Alain, Bordes, Florence
Format: Artigo
Language:Inglês
Published: John Wiley and Sons Inc. 2019
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ARTICLES
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6771796/
https://ncbi.nlm.nih.gov/pubmed/31282998
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bit.27102
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