Developing a Versatile Shotgun Cloning Strategy for Single-Vector-Based Multiplex Expression of Short Interfering RNAs (siRNAs) in Mammalian Cells

[Image: see text] As an important post-transcriptional regulatory machinery mediated by ∼21nt short-interfering double-stranded RNA (siRNA), RNA interference (RNAi) is a powerful tool to delineate gene functions and develop therapeutics. However, effective RNAi-mediated silencing requires multiple s...

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Detaylı Bibliyografya
Yayımlandı:ACS Synth Biol
Asıl Yazarlar: Wang, Xi, Yuan, Chengfu, Huang, Bo, Fan, Jiaming, Feng, Yixiao, Li, Alexander J., Zhang, Bo, Lei, Yan, Ye, Zhenyu, Zhao, Ling, Cao, Daigui, Yang, Lijuan, Wu, Di, Chen, Xian, Liu, Bin, Wagstaff, William, He, Fang, Wu, Xiaoxing, Luo, Huaxiu, Zhang, Jing, Zhang, Meng, Haydon, Rex C., Luu, Hue H., Lee, Michael J., Moriatis Wolf, Jennifer, Huang, Ailong, He, Tong-Chuan, Zeng, Zongyue
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: American Chemical Society 2019
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC6760290/
https://ncbi.nlm.nih.gov/pubmed/31465214
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acssynbio.9b00203
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