Developing a Versatile Shotgun Cloning Strategy for Single-Vector-Based Multiplex Expression of Short Interfering RNAs (siRNAs) in Mammalian Cells

[Image: see text] As an important post-transcriptional regulatory machinery mediated by ∼21nt short-interfering double-stranded RNA (siRNA), RNA interference (RNAi) is a powerful tool to delineate gene functions and develop therapeutics. However, effective RNAi-mediated silencing requires multiple s...

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Publicado no:ACS Synth Biol
Main Authors: Wang, Xi, Yuan, Chengfu, Huang, Bo, Fan, Jiaming, Feng, Yixiao, Li, Alexander J., Zhang, Bo, Lei, Yan, Ye, Zhenyu, Zhao, Ling, Cao, Daigui, Yang, Lijuan, Wu, Di, Chen, Xian, Liu, Bin, Wagstaff, William, He, Fang, Wu, Xiaoxing, Luo, Huaxiu, Zhang, Jing, Zhang, Meng, Haydon, Rex C., Luu, Hue H., Lee, Michael J., Moriatis Wolf, Jennifer, Huang, Ailong, He, Tong-Chuan, Zeng, Zongyue
Formato: Artigo
Idioma:Inglês
Publicado em: American Chemical Society 2019
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6760290/
https://ncbi.nlm.nih.gov/pubmed/31465214
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acssynbio.9b00203
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