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Measurement of Ligand Binding to Tubulin by Sulfhydryl Reactivity
Ligand binding can induce shifts in protein conformation. In the case of tubulin, these drug-induced confirmational changes can prevent or stabilize microtubule polymerization. 5′,5′-Dithiobis(2-nitrobenzoate) (DTNB) reacts with free and accessible sulfhydryls and stoichiometrically produces a detec...
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| Udgivet i: | Methods Cell Biol |
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| Main Authors: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2010
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6752050/ https://ncbi.nlm.nih.gov/pubmed/20466146 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/S0091-679X(10)95021-8 |
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