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Homology Modeling-Based in Silico Affinity Maturation Improves the Affinity of a Nanobody
Affinity maturation and rational design have a raised importance in the application of nanobody (VHH), and its unique structure guaranteed these processes quickly done in vitro. An anti-CD47 nanobody, Nb02, was screened via a synthetic phage display library with 278 nM of K(D) value. In this study,...
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| Veröffentlicht in: | Int J Mol Sci |
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| Hauptverfasser: | , , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
MDPI
2019
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6747709/ https://ncbi.nlm.nih.gov/pubmed/31461846 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms20174187 |
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