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Homology Modeling-Based in Silico Affinity Maturation Improves the Affinity of a Nanobody

Affinity maturation and rational design have a raised importance in the application of nanobody (VHH), and its unique structure guaranteed these processes quickly done in vitro. An anti-CD47 nanobody, Nb02, was screened via a synthetic phage display library with 278 nM of K(D) value. In this study,...

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Detalhes bibliográficos
Publicado no:Int J Mol Sci
Main Authors: Cheng, Xin, Wang, Jiewen, Kang, Guangbo, Hu, Min, Yuan, Bo, Zhang, Yingtian, Huang, He
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6747709/
https://ncbi.nlm.nih.gov/pubmed/31461846
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms20174187
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