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An Improved Method of Maintaining Primary Murine Cardiac Fibroblasts in Two-Dimensional Cell Culture

Primary cardiac fibroblasts are notoriously difficult to maintain for extended periods of time in cell culture, due to the plasticity of their phenotype and sensitivity to mechanical input. In order to study cardiac fibroblast activation in vitro, we have developed cell culture conditions which prom...

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Détails bibliographiques
Publié dans:Sci Rep
Auteurs principaux: Landry, Natalie M., Rattan, Sunil G., Dixon, Ian M. C.
Format: Artigo
Langue:Inglês
Publié: Nature Publishing Group UK 2019
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6733858/
https://ncbi.nlm.nih.gov/pubmed/31501457
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-49285-9
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