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Integrating SpyCatcher/SpyTag covalent fusion technology into phage display workflows for rapid antibody discovery

An early bottleneck in the rapid isolation of new antibody fragment binders using in vitro library approaches is the inertia encountered in acquiring and preparing soluble antigen fragments. In this report, we describe a simple, yet powerful strategy that exploits the properties of the SpyCatcher/Sp...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Fierle, Julie K., Abram-Saliba, Johan, Brioschi, Matteo, deTiani, Mariastella, Coukos, George, Dunn, Steven M.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6731262/
https://ncbi.nlm.nih.gov/pubmed/31492910
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-49233-7
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