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Covalent protein labeling by SpyTag-SpyCatcher in fixed cells for super-resolution microscopy

Labeling proteins with high specificity and efficiency is a fundamental prerequisite for microscopic visualization of subcellular protein structures and interactions. While the comparatively small size of epitope tags makes them less perturbative to fusion proteins, they require the use of large ant...

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Vydáno v:Chembiochem
Hlavní autoři: Pessino, Veronica, Citron, Rose, Feng, Siyu, Huang, Bo
Médium: Artigo
Jazyk:Inglês
Vydáno: 2017
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5599254/
https://ncbi.nlm.nih.gov/pubmed/28514494
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201700177
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