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Covalent protein labeling by SpyTag-SpyCatcher in fixed cells for super-resolution microscopy
Labeling proteins with high specificity and efficiency is a fundamental prerequisite for microscopic visualization of subcellular protein structures and interactions. While the comparatively small size of epitope tags makes them less perturbative to fusion proteins, they require the use of large ant...
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| Publié dans: | Chembiochem |
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| Auteurs principaux: | , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2017
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5599254/ https://ncbi.nlm.nih.gov/pubmed/28514494 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201700177 |
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