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Cell-free mixing of Escherichia coli crude extracts to prototype and rationally engineer high-titer mevalonate synthesis
Cell-free metabolic engineering (CFME) is advancing a powerful paradigm for accelerating the design and synthesis of biosynthetic pathways. However, as most cell-free biomolecule synthesis systems to date use purified enzymes, energy and cofactor supply can be limiting. To address this challenge, we...
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| Publicado en: | ACS Synth Biol |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2016
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6728267/ https://ncbi.nlm.nih.gov/pubmed/27476989 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acssynbio.6b00154 |
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