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Cell-free mixing of Escherichia coli crude extracts to prototype and rationally engineer high-titer mevalonate synthesis

Cell-free metabolic engineering (CFME) is advancing a powerful paradigm for accelerating the design and synthesis of biosynthetic pathways. However, as most cell-free biomolecule synthesis systems to date use purified enzymes, energy and cofactor supply can be limiting. To address this challenge, we...

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書誌詳細
出版年:ACS Synth Biol
主要な著者: Dudley, Quentin M., Anderson, Kim C., Jewett, Michael C.
フォーマット: Artigo
言語:Inglês
出版事項: 2016
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC6728267/
https://ncbi.nlm.nih.gov/pubmed/27476989
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acssynbio.6b00154
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