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Native Reversed-Phase Liquid Chromatography: A Technique for LCMS of Intact Antibody–Drug Conjugates

The synthesis of antibody–drug conjugates (ADCs) using the interchain cysteines of the antibody inherently gives a mixture of proteins with varying drug-to-antibody ratio. The drug distribution profiles of ADCs are routinely characterized by hydrophobic interaction chromatography (HIC). Because HIC...

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Dettagli Bibliografici
Pubblicato in:Anal Chem
Autori principali: Chen, Tse-Hong, Yang, Yun, Zhang, Zhaorui, Fu, Cexiong, Zhang, Qunying, Williams, Jon D., Wirth, Mary J.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2019
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6727645/
https://ncbi.nlm.nih.gov/pubmed/30661356
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.8b04699
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