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PTO-QuickStep: A Fast and Efficient Method for Cloning Random Mutagenesis Libraries
QuickStep is a cloning method that allows seamless point integration of a DNA sequence at any position within a target plasmid using only Q5 High-Fidelity DNA Polymerase and DpnI endonuclease. This efficient and cost-effective method consists of two steps: two parallel asymmetric PCRs, followed by a...
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| 發表在: | Int J Mol Sci |
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| Main Authors: | , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
MDPI
2019
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6720219/ https://ncbi.nlm.nih.gov/pubmed/31405219 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms20163908 |
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