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Fast and easy method for construction of plasmid vectors using modified Quick-change mutagenesis
Plasmid vector construction is an essential step for molecular microbiology yet often a time-consuming process. Manipulation of the fungal genome to express genes to activate secondary metabolite production often requires creation of plasmid constructs in a reiterative fashion. Here we introduce a m...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3692276/ https://ncbi.nlm.nih.gov/pubmed/23065615 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-62703-122-6_11 |
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