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Residues W215, E217 and E192 control the allosteric E*-E equilibrium of thrombin

A pre-existing, allosteric equilibrium between closed (E*) and open (E) conformations of the active site influences the level of activity in the trypsin fold and defines ligand binding according to the mechanism of conformational selection. Using the clotting protease thrombin as a model system, we...

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Publicado en:Sci Rep
Main Authors: Pelc, Leslie A., Koester, Sarah K., Chen, Zhiwei, Gistover, Noah E., Di Cera, Enrico
Formato: Artigo
Idioma:Inglês
Publicado: Nature Publishing Group UK 2019
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6707225/
https://ncbi.nlm.nih.gov/pubmed/31444378
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-48839-1
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