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Two-Dimensional Droplet Digital PCR as a Tool for Titration and Integrity Evaluation of Recombinant Adeno-Associated Viral Vectors

Recombinant adeno-associated virus (rAAV) vectors have recently been widely utilized for in in vivo gene therapy. The clinical dose definition of AAV vector requires the exact quantification as starting doses and for dose-escalation studies. Vector genome (vg) copies measured by quantitative PCR (qP...

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Publicado en:Hum Gene Ther Methods
Main Authors: Furuta-Hanawa, Birei, Yamaguchi, Teruhide, Uchida, Eriko
Formato: Artigo
Idioma:Inglês
Publicado: Mary Ann Liebert, Inc., publishers 2019
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6707039/
https://ncbi.nlm.nih.gov/pubmed/31140327
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/hgtb.2019.031
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