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Accurate high-throughput screening based on digital protein synthesis in a massively parallel femtoliter droplet array
We report a general strategy based on digital counting principle that enables an efficient acquisition of enzyme mutants with desired activities from just a few clones within a day. We prepared a high-density femtoliter droplet array, consisting of 1 million uniform droplets per 1 cm(2) to carry out...
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| Vydáno v: | Sci Adv |
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| Hlavní autoři: | , , , , , , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
American Association for the Advancement of Science
2019
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6703874/ https://ncbi.nlm.nih.gov/pubmed/31457078 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/sciadv.aav8185 |
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