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Engineering a Polyspecific Pyrrolysyl-tRNA Synthetase by a High Throughput FACS Screen

The Pyrrolysyl-tRNA synthetase (PylRS) and its cognate tRNA(Pyl) are extensively used to add non-canonical amino acids (ncAAs) to the genetic code of bacterial and eukaryotic cells. However, new ncAAs often require a cumbersome de novo engineering process to generate an appropriate PylRS/tRNA(Pyl) p...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Hohl, Adrian, Karan, Ram, Akal, Anastassja, Renn, Dominik, Liu, Xuechao, Ghorpade, Seema, Groll, Michael, Rueping, Magnus, Eppinger, Jörg
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6700097/
https://ncbi.nlm.nih.gov/pubmed/31427620
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-019-48357-0
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