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How to evaluate the cellular uptake of CPPs with fluorescence techniques: dissecting methodological pitfalls associated to tryptophan-rich peptides.

Cell-penetrating peptides (CPP) are broadly recognized as efficient non-viral vectors for the internalization of compounds such as peptides, oligonucleotides or proteins. Characterizing these carriers requires reliable methods to quantify their intracellular uptake. Flow cytometry on living cells is...

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Vydáno v:Biochim Biophys Acta Biomembr
Hlavní autoři: Seisel, Quentin, Pelletier, François, Deshayes, Sébastien, Boisguerin, Prisca
Médium: Artigo
Jazyk:Inglês
Vydáno: 2019
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6689431/
https://ncbi.nlm.nih.gov/pubmed/31283917
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbamem.2019.06.011
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