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How to evaluate the cellular uptake of CPPs with fluorescence techniques: dissecting methodological pitfalls associated to tryptophan-rich peptides.

Cell-penetrating peptides (CPP) are broadly recognized as efficient non-viral vectors for the internalization of compounds such as peptides, oligonucleotides or proteins. Characterizing these carriers requires reliable methods to quantify their intracellular uptake. Flow cytometry on living cells is...

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Detalhes bibliográficos
Publicado no:Biochim Biophys Acta Biomembr
Main Authors: Seisel, Quentin, Pelletier, François, Deshayes, Sébastien, Boisguerin, Prisca
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6689431/
https://ncbi.nlm.nih.gov/pubmed/31283917
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbamem.2019.06.011
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