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Design of thiazole orange oligonucleotide probes for detection of DNA and RNA by fluorescence and duplex melting

We have synthesised a range of thiazole orange (TO) functionalised oligonucleotides for nucleic acid detection in which TO is attached to the nucleobase or sugar of thymidine. The properties of duplexes between TO-probes and their DNA and RNA targets strongly depend on the length of the linker betwe...

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Detalles Bibliográficos
Publicado en:Org Biomol Chem
Autores principales: Klimkowski, Piotr, De Ornellas, Sara, Singleton, Daniel, El-Sagheer, Afaf H., Brown, Tom
Formato: Artigo
Lenguaje:Inglês
Publicado: Royal Society of Chemistry 2019
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC6686645/
https://ncbi.nlm.nih.gov/pubmed/31157811
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c9ob00885c
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