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Fluorogenic thiazole orange TOTFO probes stabilise parallel DNA triplexes at pH 7 and above

The instability of DNA triplexes particularly at neutral pH and above severely limits their applications. Here, we demonstrate that the introduction of a thiazole orange (TO) intercalator onto a thymine nucleobase in triplex forming oligonucleotides (TFOs) resolves this problem. The stabilising effe...

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Detalhes bibliográficos
Publicado no:Chem Sci
Main Authors: Walsh, Sarah, El-Sagheer, Afaf Helmy, Brown, Tom
Formato: Artigo
Idioma:Inglês
Publicado em: Royal Society of Chemistry 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6182420/
https://ncbi.nlm.nih.gov/pubmed/30393529
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c8sc02418a
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