A Convenient Thiazole Orange Fluorescence Assay for the Evaluation of DNA Duplex Hybridization Stability

OBJECTIVE: A simple and rapid method for measuring the hybridization stability of duplexes of DNAs and other oligomers in different environments is described. When added to an oligomer duplex, the thiazole orange (TO) dye intercalates and in this state is fluorescent. Therefore, when duplex dissocia...

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Vydáno v:Mol Imaging Biol
Hlavní autoři: Liang, Minmin, Liu, Xinrong, Nakamura, Kayoko, Chen, Xiangji, Cheng, Dengfeng, Liu, Guozheng, Dou, Shuping, Wang, Yi, Rusckowski, Mary, Hnatowich, Donald J.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2009
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4797636/
https://ncbi.nlm.nih.gov/pubmed/19444399
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s11307-009-0221-4
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