Proteome Profiling of 1 to 5 Spiked Circulating Tumor Cells Isolated from Whole Blood Using Immunodensity Enrichment, Laser Capture Microdissection, Nanodroplet Sample Processing, and Ultrasensitive nanoLC-MS

Proteome profiling of circulating tumor cells (CTCs) can provide crucial insight into disease progression and the role of CTCs in tumor metastasis. We describe an integrated workflow to measure global protein expression in 1–5 spiked CTCs enriched from whole blood by immunodensity gradient centrifug...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Anal Chem
Prif Awduron: Zhu, Ying, Podolak, Jennifer, Zhao, Rui, Shukla, Anil K., Moore, Ronald J., Thomas, George V., Kelly, Ryan T.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2018
Pynciau:
Article
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC6686195/
https://ncbi.nlm.nih.gov/pubmed/30269481
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.8b03268
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