Proteomic Analysis of Single Mammalian Cells Enabled by Microfluidic Nanodroplet Sample Preparation and Ultrasensitive NanoLC-MS

We report on the quantitative proteomic analysis of single mammalian cells. Fluorescence-activated cell sorting was employed to deposit cells into a newly developed nano-droplet sample processing chip, after which samples were analyzed by ultrasensitive nanoLC-MS. An average of circa 670 protein gro...

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Detalhes bibliográficos
Publicado no:Angew Chem Int Ed Engl
Main Authors: Zhu, Ying, Clair, Geremy, Chrisler, William B., Shen, Yufeng, Zhao, Rui, Shukla, Anil K., Moore, Ronald J., Misra, Ravi S., Pryhuber, Gloria S., Smith, Richard D., Ansong, Charles, Kelly, Ryan T.
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6261339/
https://ncbi.nlm.nih.gov/pubmed/29797682
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201802843
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