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CRISPR/Cas9‐enhanced ssDNA recombineering for Pseudomonas putida

Implementation of single‐stranded DNA (ssDNA) recombineering in Pseudomonas putida has widened the range of genetic manipulations applicable to this biotechnologically relevant bacterium. Yet, the relatively low efficiency of the technology hampers identification of mutated clones lacking conspicuou...

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Publicat a:Microb Biotechnol
Autors principals: Aparicio, Tomás, de Lorenzo, Víctor, Martínez‐García, Esteban
Format: Artigo
Idioma:Inglês
Publicat: John Wiley and Sons Inc. 2019
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6681395/
https://ncbi.nlm.nih.gov/pubmed/31237429
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/1751-7915.13453
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