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High-Efficiency Multi-site Genomic Editing of Pseudomonas putida through Thermoinducible ssDNA Recombineering

Application of single-stranded DNA recombineering for genome editing of species other than enterobacteria is limited by the efficiency of the recombinase and the action of endogenous mismatch repair (MMR) systems. In this work we have set up a genetic system for entering multiple changes in the chro...

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Detaylı Bibliyografya
Yayımlandı:iScience
Asıl Yazarlar: Aparicio, Tomas, Nyerges, Akos, Martínez-García, Esteban, de Lorenzo, Víctor
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Elsevier 2020
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC7068128/
https://ncbi.nlm.nih.gov/pubmed/32179472
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.isci.2020.100946
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