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Using Recombineering to Generate Point Mutations: Oligonucleotide-based “Hit & Fix” Method
Ability to manipulate the genome or design genes with desired mutation is critical for functional studies. Recombineering has made genetic manipulation of large genomic fragments very feasible and efficient. In the bacteriophage lambda-based recombineering system, three prophage genes, exo, bet, and...
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| Publicado no: | Methods Mol Biol |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6668621/ https://ncbi.nlm.nih.gov/pubmed/22328429 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-61779-564-0_9 |
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