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Using Recombineering to Generate Point Mutations: galK-Based Positive-Negative Selection Method

Recombineering is a recombination-based highly efficient method of genetic engineering. It can be used to manipulate the bacterial chromosomal DNA as well as any episomal DNA. Recombineering can be used to insert selectable or non-selectable DNA fragments, subclone DNA fragments without the use of r...

詳細記述

保存先:
書誌詳細
出版年:Methods Mol Biol
主要な著者: Biswas, Kajal, Stauffer, Stacey, Sharan, Shyam K.
フォーマット: Artigo
言語:Inglês
出版事項: 2012
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC6668620/
https://ncbi.nlm.nih.gov/pubmed/22328430
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-61779-564-0_10
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