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Using Recombineering to Generate Point Mutations: galK-Based Positive-Negative Selection Method
Recombineering is a recombination-based highly efficient method of genetic engineering. It can be used to manipulate the bacterial chromosomal DNA as well as any episomal DNA. Recombineering can be used to insert selectable or non-selectable DNA fragments, subclone DNA fragments without the use of r...
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| Veröffentlicht in: | Methods Mol Biol |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2012
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6668620/ https://ncbi.nlm.nih.gov/pubmed/22328430 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-61779-564-0_10 |
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