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Using Recombineering to Generate Point Mutations: galK-Based Positive-Negative Selection Method

Recombineering is a recombination-based highly efficient method of genetic engineering. It can be used to manipulate the bacterial chromosomal DNA as well as any episomal DNA. Recombineering can be used to insert selectable or non-selectable DNA fragments, subclone DNA fragments without the use of r...

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Bibliografski detalji
Izdano u:Methods Mol Biol
Glavni autori: Biswas, Kajal, Stauffer, Stacey, Sharan, Shyam K.
Format: Artigo
Jezik:Inglês
Izdano: 2012
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6668620/
https://ncbi.nlm.nih.gov/pubmed/22328430
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-61779-564-0_10
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