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A high-throughput mass spectrometric enzyme activity assay enabling the discovery of cytochrome P450 biocatalysts

Assaying for enzymatic activity is a persistent bottleneck in biocatalyst and drug development. Existing high-throughput assays for enzyme activity tend to be applicable only to a narrow range of biochemical transformations, whereas universal enzyme characterization methods usually require chromatog...

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Bibliografiske detaljer
Udgivet i:Angew Chem Int Ed Engl
Main Authors: de Rond, Tristan, Gao, Jian, Zargar, Amin, de Raad, Markus, Cunha, Jack, Northen, Trent R., Keasling, Jay D.
Format: Artigo
Sprog:Inglês
Udgivet: 2019
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6640108/
https://ncbi.nlm.nih.gov/pubmed/31140688
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201901782
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