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Demonstration of highly efficient dual gRNA CRISPR/Cas9 editing of the homeologous GmFAD2–1A and GmFAD2–1B genes to yield a high oleic, low linoleic and α-linolenic acid phenotype in soybean

BACKGROUND: CRISPR/Cas9 gene editing is now revolutionizing the ability to effectively modify plant genomes in the absence of efficient homologous recombination mechanisms that exist in other organisms. However, soybean is allotetraploid and is commonly viewed as difficult and inefficient to transfo...

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التفاصيل البيبلوغرافية
الحاوية / القاعدة:	BMC Plant Biol
المؤلفون الرئيسيون:	Do, Phat T., Nguyen, Cuong X., Bui, Hien T., Tran, Ly T. N., Stacey, Gary, Gillman, Jason D., Zhang, Zhanyuan J., Stacey, Minviluz G.
التنسيق:	Artigo
اللغة:	Inglês
منشور في:	BioMed Central 2019
الموضوعات:	Research Article
الوصول للمادة أونلاين:	https://ncbi.nlm.nih.gov/pmc/articles/PMC6632005/ https://ncbi.nlm.nih.gov/pubmed/31307375 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12870-019-1906-8
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Demonstration of highly efficient dual gRNA CRISPR/Cas9 editing of the homeologous GmFAD2–1A and GmFAD2–1B genes to yield a high oleic, low linoleic and α-linolenic acid phenotype in soybean

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