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Recombinant production of ESAT-6 antigen in thermoinducible Escherichia coli: the role of culture scale and temperature on metabolic response, expression of chaperones, and architecture of inclusion bodies

The heat-inducible expression system has been widely used to produce recombinant proteins in Escherichia coli. However, the rise in temperature affects cell growth, activates the bacterial Heat-Shock Response (HSR), and promotes the formation of insoluble protein aggregates known as inclusion bodies...

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Bibliographic Details
Published in:Cell Stress Chaperones
Main Authors: Restrepo-Pineda, Sara, Bando-Campos, Carlos G., Valdez-Cruz, Norma A., Trujillo-Roldán, Mauricio A.
Format: Artigo
Language:Inglês
Published: Springer Netherlands 2019
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6629757/
https://ncbi.nlm.nih.gov/pubmed/31165436
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12192-019-01006-x
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