Production of a recombinant phospholipase A2 in Escherichia coli using resonant acoustic mixing that improves oxygen transfer in shake flasks

BACKGROUND: Shake flasks are widely used during the development of bioprocesses for recombinant proteins. Cultures of recombinant Escherichia coli with orbital mixing (OM) have an oxygen limitation negatively affecting biomass growth and recombinant-protein production. With the aim to improve mixing...

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Detalhes bibliográficos
Publicado no:Microb Cell Fact
Main Authors: Valdez-Cruz, Norma A., Reynoso-Cereceda, Greta I., Pérez-Rodriguez, Saumel, Restrepo-Pineda, Sara, González-Santana, Jesus, Olvera, Alejandro, Zavala, Guadalupe, Alagón, Alejandro, Trujillo-Roldán, Mauricio A.
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2017
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5526256/
https://ncbi.nlm.nih.gov/pubmed/28743267
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-017-0746-1
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