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Quantifying acute fuel and respiration dependent pH homeostasis in live cells using the mCherryTYG mutant as a fluorescence lifetime sensor.

Intracellular pH plays a key role in physiology, and its measurement in living specimens remains a crucial task in biology. Fluorescent protein-based pH sensors have gained widespread use, but there is limited spectral diversity for multicolor detection, and it remains a challenge to measure absolut...

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Bibliografske podrobnosti
izdano v:Anal Chem
Main Authors: Haynes, Emily P., Rajendran, Megha, Henning, Chace K., Mishra, Abhipri, Lyon, Angeline M., Tantama, Mathew
Format: Artigo
Jezik:Inglês
Izdano: 2019
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6623984/
https://ncbi.nlm.nih.gov/pubmed/31247720
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.9b01562
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