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Quantifying acute fuel and respiration dependent pH homeostasis in live cells using the mCherryTYG mutant as a fluorescence lifetime sensor.
Intracellular pH plays a key role in physiology, and its measurement in living specimens remains a crucial task in biology. Fluorescent protein-based pH sensors have gained widespread use, but there is limited spectral diversity for multicolor detection, and it remains a challenge to measure absolut...
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| Publié dans: | Anal Chem |
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| Auteurs principaux: | , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2019
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6623984/ https://ncbi.nlm.nih.gov/pubmed/31247720 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.9b01562 |
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