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A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo
To expand the toolbox of imaging in living cells, we have engineered a single-chain variable fragment binding the linear HA epitope with high affinity and specificity in vivo. The resulting probe, called the HA frankenbody, can light up in multiple colors HA-tagged nuclear, cytoplasmic, membrane, an...
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| Publicado no: | Nat Commun |
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| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6610143/ https://ncbi.nlm.nih.gov/pubmed/31270320 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-019-10846-1 |
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