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Reconstitution and biochemical characterization of ribonucleoprotein complexes in Type I-E CRISPR-Cas systems
Type I CRISPR-Cas, the most prevalent CRISPR system, features a sequential target searching and degradation process. First, the multi-subunit surveillance complex Cascade recognizes the matching dsDNA target flanked by protospacer adjacent motif (PAM), promotes the heteroduplex formation between CRI...
Gardado en:
| Publicado en: | Methods Enzymol |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2018
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6607021/ https://ncbi.nlm.nih.gov/pubmed/30691647 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2018.11.003 |
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