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Optimizing Systems for Cas9 Expression in Toxoplasma gondii

CRISPR-Cas9 technologies have enabled genome engineering in an unprecedented array of species, accelerating biological studies in both model and nonmodel systems. However, Cas9 can be inherently toxic, which has limited its use in some organisms. We previously described the serendipitous discovery o...

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發表在:mSphere
Main Authors: Markus, Benedikt M., Bell, George W., Lorenzi, Hernan A., Lourido, Sebastian
格式: Artigo
語言:Inglês
出版: American Society for Microbiology 2019
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC6595152/
https://ncbi.nlm.nih.gov/pubmed/31243081
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mSphere.00386-19
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