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Optimizing Systems for Cas9 Expression in Toxoplasma gondii

CRISPR-Cas9 technologies have enabled genome engineering in an unprecedented array of species, accelerating biological studies in both model and nonmodel systems. However, Cas9 can be inherently toxic, which has limited its use in some organisms. We previously described the serendipitous discovery o...

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Bibliographic Details
Published in:mSphere
Main Authors: Markus, Benedikt M., Bell, George W., Lorenzi, Hernan A., Lourido, Sebastian
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2019
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6595152/
https://ncbi.nlm.nih.gov/pubmed/31243081
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mSphere.00386-19
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