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Optimizing Systems for Cas9 Expression in Toxoplasma gondii

CRISPR-Cas9 technologies have enabled genome engineering in an unprecedented array of species, accelerating biological studies in both model and nonmodel systems. However, Cas9 can be inherently toxic, which has limited its use in some organisms. We previously described the serendipitous discovery o...

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Detalles Bibliográficos
Publicado en:mSphere
Main Authors: Markus, Benedikt M., Bell, George W., Lorenzi, Hernan A., Lourido, Sebastian
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2019
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6595152/
https://ncbi.nlm.nih.gov/pubmed/31243081
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mSphere.00386-19
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