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Optimizing Systems for Cas9 Expression in Toxoplasma gondii

CRISPR-Cas9 technologies have enabled genome engineering in an unprecedented array of species, accelerating biological studies in both model and nonmodel systems. However, Cas9 can be inherently toxic, which has limited its use in some organisms. We previously described the serendipitous discovery o...

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Detalhes bibliográficos
Publicado no:mSphere
Main Authors: Markus, Benedikt M., Bell, George W., Lorenzi, Hernan A., Lourido, Sebastian
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6595152/
https://ncbi.nlm.nih.gov/pubmed/31243081
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mSphere.00386-19
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