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(31)P and (1)H NMR investigation of the structure of the phosphorylating intermediates in the phosphodiester approach to the oligonucleotide synthesis
The structure of the reactive phosphorylating derivatives (B) formed by treatment of pdT(Ac) and of the model compound phenylphosphate with condensing reagents in pyridine was studied. Using γ-picoline instead of pyridine it was demonstrated by phosphorus and proton NMR spectroscopy that B contains...
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| Publicado no: | Nucleic Acids Res |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1978
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6581364/ https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/1.suppl_1.s209 |
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