Improved CRISPR/Cas9 gene editing by fluorescence activated cell sorting of green fluorescence protein tagged protoplasts

BACKGROUND: CRISPR/Cas9 is widely used for precise genetic editing in various organisms. CRISPR/Cas9 editing may in many plants be hampered by the presence of complex and high ploidy genomes and inefficient or poorly controlled delivery of the CRISPR/Cas9 components to gamete cells or cells with reg...

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Bibliographic Details
Published in:BMC Biotechnol
Main Authors: Petersen, Bent Larsen, Möller, Svenning Rune, Mravec, Jozef, Jørgensen, Bodil, Christensen, Mikkel, Liu, Ying, Wandall, Hans H., Bennett, Eric Paul, Yang, Zhang
Format: Artigo
Language:Inglês
Published: BioMed Central 2019
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6580576/
https://ncbi.nlm.nih.gov/pubmed/31208390
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-019-0530-x
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