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RNAi in Dictyostelium: The Role of RNA-directed RNA Polymerases and Double-stranded RNase

We show that in Dictyostelium discoideum an endogenous gene as well as a transgene can be silenced by introduction of a gene construct that is transcribed into a hairpin RNA. Gene silencing was accompanied by the appearance of sequence-specific RNA ∼23mers and seemed to have a limited capacity. The...

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Bibliografski detalji
Glavni autori: Martens, Henrik, Novotny, Jindrich, Oberstrass, Jürgen, Steck, Theodore L., Postlethwait, Pamela, Nellen, Wolfgang
Format: Artigo
Jezik:Inglês
Izdano: The American Society for Cell Biology 2002
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC65640/
https://ncbi.nlm.nih.gov/pubmed/11854403
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.01-04-0211
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