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High-throughput mapping of CoA metabolites by SAMDI-MS to optimize the cell-free biosynthesis of HMG-CoA

Metabolic engineering uses enzymes to produce small molecules with industrial, pharmaceutical, and energy applications. However, efforts to optimize enzymatic pathways for commercial production are limited by the throughput of assays for quantifying metabolic intermediates and end products. We devel...

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Detalhes bibliográficos
Publicado no:Sci Adv
Main Authors: O’Kane, Patrick T., Dudley, Quentin M., McMillan, Aislinn K., Jewett, Michael C., Mrksich, Milan
Formato: Artigo
Idioma:Inglês
Publicado em: American Association for the Advancement of Science 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6551189/
https://ncbi.nlm.nih.gov/pubmed/31183410
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/sciadv.aaw9180
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