Optimization, validation and application of an assay for the activity of HMG-CoA reductase in vitro by LC–MS/MS()

A stable HMG-CoA reductase (HMGR) reaction in vitro was developed by a sensitive, selective and precise liquid chromatography–tandem mass spectrometry (LC–MS/MS) method. The optimized enzyme reaction condition contained 1.5 μg of HMGR, 20 nM of NADPH with 50 min of reaction time. The method was vali...

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Detalhes bibliográficos
Publicado no:J Pharm Anal
Main Authors: Wang, Jing, Sun, Ji-Ye, Sha, Chun-Jie, Shao, Yu-Feng, Liu, Yan-Hong, Li, You-Xin, Duan, Zhen-Wen, Liu, Wan-Hui
Formato: Artigo
Idioma:Inglês
Publicado em: Xi'an Jiaotong University 2015
Assuntos:
Original Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5762244/
https://ncbi.nlm.nih.gov/pubmed/29403953
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jpha.2015.06.002
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